Technological Scope

“Between atom and cell”

ARBRE focuses on molecular-scale biophysics.

This is a dynamic interdisciplinary field that aims to study biological macromolecules and assemblies as a whole, at an intermediate level between atomic-resolution structural descriptions and cellular-level observations (“Between atom and cell”), with significant applications in biomedicine and drug discovery.

It has a well-defined identity of its own, which interfaces with several other closely-related methodological fields.

ARBRE aims to ally and synergize the power
of a wide diversity of technological approaches.

Technologies of expertise of ARBRE members
(non-exhaustive and continuously evolving list)

Advanced spectroscopies

  • CD: Circular Dichroism
  • EPR: Electron Paramagnetic Resonance
  • Fluorescence Microscopy (FRET, FLIM, FCS, …)
  • Fluorescence Spectroscopy (including fluorescence anisotropy)
  • FTIR: Fourier Transform Infrared Spectroscopy
  • LD: Linear Dichroism
  • Mössbauer spectroscopy
  • MST: Micro Scale Thermophoresis
  • Raman spectroscopy

Hydrodynamics

  • AUC: Analytical Ultra Centrifugation
  • DLS: Dynamic Light Scattering
  • FIDA: Flow-Induced Dispersion Analysis
  • MDS: Microfluidic Diffusional Sizing
  • SAXS: Small Angle X-ray Scattering
  • SEC-MALS: Size Exclusion Chromatography – Multi-Angle Light Scattering
  • Second Harmonic Scattering

Real-time Biosensing

  • BLI: BioLayer Interferometry
  • GCI: Grating-Coupled Interferometry
  • QCM: Quartz Crystal Microbalance
  • Rapid Kinetic approaches: Stopped and continuous flow, T-Jump, Rapid freeze-quench
  • SPR: Surface Plasmon Resonance

Single-Molecule approaches

  • AFM: Atomic Force Microscopy
  • Magnetic tweezers
  • MP: Mass Photometry
  • Optical Tweezers
  • Single-molecule FRET

Thermodynamics

  • DSC: Differential Scanning Calorimetry
  • DSF: Differential Scanning Fluorimetry
  • ITC: Isothermal Titration Calorimetry

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